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PUBLICATIONS

2019

66. Topcu, E. and Biggar, K.K. (2019) PeSA: A software tool for peptide specificity analysis. Computat. Biol. Chem. 83, 107145.

65. Chopra, A., Willmore, W. and Biggar, K.K. (2019) Protein quantification and visualization via ultraviolet-dependent labeling with 2,2,2-trichloroethanol. Sci. Rep. 9(1), 1-8.

64. Al-attar, R., Wu, C.W., Biggar, K.K. and Storey, K. (2019) Carb-loading: Freeze-induced activation of the glucose-respnsive ChREBP transcriptional network in wood frogs. Physiol. Biochem. Zool. In press. DOI: https://doi.org/10.1086/706463

63. Tri, L.D., Childers, C.L., Adam, M.K., Ben, R.N., Storey, K.B. and Biggar, K.K. (2019) Characterization of ice recrystalization inhibition activity in the novel freeze-responsive protein Fr10 from freeze-tolerant wood frogs, Rana sylvatica. J. Themal Biol. 84, 426-430.

62. Mezey, N., Cho, W. and Biggar, K.K. (2019) The intriguing origins of protein lysine methylation: influencing cell function through dynamic methylation of non-histone proteins. Genom. Proteom. Bioinform. Accepted. GPB-D-00206.

 

61. Adhikary, H., Bakos, O. and Biggar, K.K. (2019) The role of protein lysine methylation in the regulation of protein function – looking beyond the histone code. In The DNA, RNA, and histone methylomes (eds. Jurga, S. and Barciszewski, J.), pp. 453-477. Springer, Cham.

 

60. Grigg, N., Schoenrock, A., Dick, K., Green, J.R., Golshani, A., Wong, A., Dehne, F., Tsai, E.C. and Biggar, K.K. (2019) Insights into the suitability of utilizing brown rats as a model for healing spinal cord injury with epidermal growth factor and fibroblast growth factor-II by predicting protein-protein interactions. Comp. Biol. Med. 104, 220-226.

 

59. Rowe, E.M., Xing, V. and Biggar, K.K. (2019) Lysine methylation: implications in neurodegenerative disease. Brain Res. 1707, 164-171.

 

58. Burnside, D., Schoenrock, A., Moteshareie, H., Hooshyar, M., Basra, P., Hajikarimloo, M., Dick, K., Barnes, B., Kazmirchuck, T., Jessulat, M., Pitre, S., Samanfar, B., Babu, M., Green, J.R., Wong, A., Dehne, F., Biggar, K.K. and Golshani, A. (2019) A robust computational tool for engineering synthetic binding proteins from random amino acid sequences. iScience 11, 375-387.

 

2018

 

57.  Singal, S.S., Biggar, K.K.*, Nygard, K.*, Shehab, M.A., S.S.C. Li, Jansson, T. and Gupta, M.B (2018) IUGR is associated with marked hyperphosphorylation of decidual and maternal plasma IGFBP-1. J. Clin. Endo. Metabol. 104(2), 408-422.

 

56. Biggar, K.K., Luu, B.E., Wu, C.W., Pifferi, F., Perret M. and Storey, K.B. (2018) Identification of novel and conserved microRNA and their expression in the gray mouse lemur, Microcebus murinus, a primate capable of daily torpor.Gene 677, 332-339.

 

55. Perry, B.W., et al.  (2018) Molecular adaptations for sensing and securing prey and insight into amniote genome diversity from the garter snake genome. Genome Biol. Evol. 10(8), 2110-2129.

 

54. Bhojoo, U. and Biggar, K.K. (2018) Single-step Purification of intrinsic protein complexes in Saccharomyces cerevisiae using regenerable calmodulin resin. MethodsX 5, 613-619.

 

53. Biggar, K.K. and Storey, K.B. The evaluation of the DNA binding affinity and protein composition of active transcription factor complexes. PeerJ. 6, e4755

 

52. Balasuriya, N., Kunkel, M.T., Biggar, K.K., Liu X., Li S.S.C., Newton, A.C., and O’Donoghue, P. Genetic code expansion and live cell imaging reveal that Thr-308 phosphorylation in the protein kinase Akt is essential and sufficient for Akt activity. J. Biol. Chem. Accepted. RA118. 002357

 

51. Rowe, E.M. and Biggar, K.K.An optimized method using peptide arrays for the identification of in vitro substrates of lysine methyltransferase enzymes. MethodsX. 5, 118-124.

 

50. Biggar, K.K., and Storey, K.B. Functional impact of non-coding RNA regulation in extreme stress adaptation. J. Mol. Cell. Biol. 10 (2), 93-101

 

 

2017

 

49. Biggar, K.K., Wang Z. and Li, S. SnapShot: Lysine methylation beyond histones. Mol. Cell 68(5):1016–1016.e1. Invited submission.

 

48. Singal, S.S., Nygard, K.*, Biggar, K.K.*, Shehab, M.A., S.S.C. Li,  Jansson, T. and Gupta, M.B.  Interaction between IGFBP-1, protein kinase CSNK-2 and mTOR in HepG2 cells as demonstrated by dual immunofluorescence and in situ PLA. Am. J. Pathol. 188(1), 111-124.

 

47. Biggar, K.K., Dawson, N.J. and Storey, K.B. Native protein denaturation using urea. Biotechniques 62(1): xiii (epub).

 

46. Shehab, M.A., Biggar, K.K., Singal, S.S., Nygard, K., Li, S.S.C., Jansson, T. and Gupta, M.B. Exposure of decidualized HESC to low oxygen tension and leucine deprivation results in increased IGFBP-1 phosphorylation and reduced IGF-I bioactivity. Mol. Cell. Endo. 452:1-14.

 

45. Moddridge, J., Biggar, K.K., Dawson, N. and Storey, K.B. Determinants of protein stability: Sensitive detection of immunoglobulin G stability using in real-time isothermal differential scanning fluorimetry. Technol. Cancer Res. Treat. 16(6): 997-1005. 

 

44. Wu, Z., Connolly, J. and Biggar, K.K. Beyond histones: The expanding roles of lysine methylation. FEBS J. 284(17): 2732-2744. 

 

43. Adema, CM, Hillier LW, Jones, CS, Loker, ES, Knight, M, Minx, P, Oliveira, G, Raghavan, N, Shedlock, A, Amaral, LR, Arican-Goktas, HD, Assis, J, Baba, EH, Baron, OL, Bayne, CJ, Benkendorff, K, Bickham-Wright, U, Biggar, KK, et al. Whole genome analysis of Biomphalaria glabrata (Mollusca, Lophotrochozoa), a snail intermediate host for transmission of schistosomiasis. Nature Comm. 8:15451. 

 

 

2016

 

42. Bansal, S., Biggar, K.K., Krivoruchko, A. and Storey, K.B. Response of the JAK-STAT signaling pathway to oxygen deprivation in the red-eared slider turtle, Trachemys scripta elegans. Gene 593(1): 34-40.

 

41. Biggar, K.K.*, Luu, B.E.*, Wu, C.W. and Storey, K.B. Torpor-responsive expression of novel microRNA regulating metabolism and other cellular pathways in the thirteen-lined ground squirrel, Ictidomys tridecemlineatus. FEBS Letters 590(20): 3574-3582.

 

40. Biggar, K.K. and Storey, K.B. Exploration of low-temperature microRNA function in an anoxia tolerant vertebrate ectotherm, the red-eared slider turtle (Trachemys scripta elegans). J. Thermal Biol. In Press.

 

39. Wu, C.W., Biggar, K.K., Luu, B.E., Szereszewski, K.E. and Storey, K.B. Analysis of microRNA expression during the torpor-arousal cycle of a mammalian hibernator, the 13-lined ground squirrel. Physiol. Genomics DOI: 10.1152/physiolgenomics.00005.2016.

 

38. Schaap, P., Barrantes, I., Minx, P., Sasaki, N., Anderson, R.W., Benard, M., Biggar, K.K., et al. The Physarum polycephalum genome reveals extensive use of prokaryotic two-component and metazoan-type tyrosine kinase signaling. Genome Biol. Evol. 8(1): 109-125.

         

37. Damerill, I., Biggar, K.K., Shehab, M.A., Li, S.S.C., Jansson, T. and Gupta M.B. Hypoxia increases IGFBP-1 phosphorylation mediated by mTOR inhibition. Mol. Endo. 30(2): 201-216.

 

36. Malkani, N., Biggar, K.K., Shehab, M.A., Li, S.S.C., Jansson, T. and Gupta M.B. Increased IGFBP-1 phosphorylation in response to leucine deprivation is mediated by CK2 and PKC. Mol. Cell. Endo.  425: 48-60.

 

 

2015

 

35. Biggar, K.K., and Storey, K.B. Low-temperature microRNA expression in the painted turtle, Chrysemys picta during freezing stress. 589(23): 3665-3670.

 

34. Peace, R., Biggar, K.K., Storey, K.B. and Green, J. A framework for improving microRNA prediction in non-human genomes. Nucl. Acid. Res. 43(20): e138.

 

33. Biggar, K.K., Sullivan, K.J. and Storey, K.B. Expression and characterization of the novel gene fr47 during freezing, anoxia, and dehydration in the wood frog, Rana sylvatica. Biochem. Res. Int. BCRI-363912.

 

32. Biggar, K.K., Wu, C.W., Tessier, S.N., Zhang, J., Pifferi, F., Perret, M., and Storey K.B. Primate torpor: Regulation of stress-activated protein kinases during daily torpor in the grey mouse lemur, Microcebus murinus. Genomics, Proteomics and Bioinformatics. 13(2): 81-90. *Special Issue on Primate hibernation.

 

31. Tessier, S.N., Zhang, J., Biggar, K.K., Wu, C.W., Pifferi F., Perret, M. and Storey, K.B. The regulation of the PI3K/AKT pathway during primate torpor in the grey mouse lemur, Microcebus murinus. Genomics, Proteomics and Bioinformatics. 13(2): 91-102. *Special Issue on Primate hibernation.

 

30. Zhang, J., Tessier, S.N., Biggar, K.K., Wu, C.W., Pifferi, F., Perret, M. and Storey, K.B. Regulation of torpor in the gray mouse lemur: transcriptional and translational controls and role of AMPK signaling. Genomics, Proteomics and Bioinformatics. 13(2): 103-110. *Special Issue on Primate hibernation.

 

29. Biggar, K.K., Wu, C.W., Tessier, S.N., Zhang, J., Pifferi, F., Perret, M. and Storey, K.B. Modulation of gene expression in key survival pathways during periods of torpor in the grey mouse lemur, Microcebus murinus. Genomics, Proteomics and Bioinformatics. 13(2): 111-118. *Special Issue on Primate hibernation. 

         

28. Biggar, K.K., Wu, C.W., Tessier, S.T., Zhang, J., Pifferi, F., Perret, M., and Storey, K.B. Induction of antioxidant and heat shock protein responses during periods of primate torpor in the mouse lemur, Microcebus murinus. Genomics, Proteomics and Bioinformatics. 13(2): 103-110. *Special Issue on Primate hibernation.

 

27. Biggar, K.K., Biggar, Y. and Storeym K.B. (2015) Identification of a novel dehydration responsive gene, drp10, from the African clawed frog, Xenopus laevis. J. Exp. Biol. A. 323(6): 375-381.

 

26. Biggar, K.K. and Storey, K.B. (2015). Insight into post-transcriptional gene regulation: Stress-responsive microRNA and their role in the survival of tolerant animals to environmental stress. J. Exp. Biol. 218(9): 1281-1289. Invited review.

 

25. Sullivan, K.J., Biggar, K.K. and Storey, K.B. (2015) Transcript expression of the freeze responsive gene fr10 in Rana sylvatica during freezing, anoxia, dehydration and development. Mol. Cell. Biochem. 399(1-2): 17-25. 

 

24. Biggar, K.K. and Li, S.S.C. (2014) Non-histone protein methylation: An emerging regulator of cellular function. Nat. Rev. Mol. Cell. Biol. 16(1): 5-17.

 

 

2014

 

23. Wu, C.W., Biggar, K.K. and Storey, K.B. (2014) Expression profiling and structural characterization of microRNAs in adipose tissues of hibernating ground squirrels. Genom. Proteom. Bioinform. 12(6): 284-291.

 

22. Biggar, K.K. and Storey, K.B. (2014) New approaches to comparative and animal stress biology research in the post-genomic era. Computat. Struct. Biotech. 11: 138-146. 

 

21. Biggar, K.K. and Storey, K.B. (2014) Insight into temperature-dependent microRNA function in mammalian hibernators: Perspectives on cold-influenced microRNA/target interaction. Temperature 1(2): 84-86. Invited Review. 

 

20. Biggar, K.K., Wu, C.W. and Storey, K.B. (2014) High-throughput amplification of mature microRNA in uncharacterized animal models using polyadenylated RNA and stem-loop RT-PCR. Anal. Biochem. 462(1): 32-34. 

 

19. Biggar, K.K. and Storey, K.B. (2014) Identification and expression of microRNA in the brain of hibernating bats, Myotis lucifugus. Gene. 544(1): 67-74. 

 

18. Rouble, A.N., Biggar, K.K. and Storey, K.B. (2014) A high-throughput protocol for message RNA quantification using RNA dot-blots. Anal. Biochem. 452(1): 31-33. 

 

 

2013

 

17. Wu, C.W., Biggar, K.K. and Storey, K.B. (2013) MicroRNA regulation during dehydration in the Western clawed frog, Xenopus tropicalis. Gene. 529(2): 269-275. 

 

16. Biggar, K.K., Dawson, N.J. and Storey, K.B. (2013) Proteomic characterization of fructose-1,6-bisphosphate aldolase from anoxia tolerance Trachemys scripta elegans: Bioinformatics and mass spectrometric exploration of enzymatic activity. PLoS ONE. 8(7): e68830. 

 

15. Biggar, K.K., Kotani, E., Furusawa, T. and Storey, K.B. (2013) Expression of freeze responsive proteins, Fr10 and Li16, from freeze tolerant frogs enhances survival of BmN insect cells. FASEB J. 27(8): 3376-3383. 

 

14. Shaffer, B.H., Minx, P., Warren, D.E., Shedlock, A.M., Thomson, R.C., Valenzuela, N., Abramyan, J., Badenhorst, D., Biggar, K.K., et al. (2013) The painted turtle genome: extreme physiological adaptations in a slowly evolving lineage. Genome Res. 14: R28. 

 

13. Wu, C.W., Biggar, K.K. and Storey, K.B. (2013) Biochemical adaptations of mammalian hibernation: exploring the thirteen-lined ground squirrel as a perspective model for naturally induced reversible insulin resistance. Braz. J. Med. Biol. Res. 46(1): 1-13. 

 

12. Zhang, J., Biggar, K.K. and Storey, K.B. (2013) Regulation of p53 by reversible post-transcriptional and post translational mechanisms in liver and skeletal muscle of anoxia tolerant turtle, Trachemys scripta elegans. Gene. 513(1): 147-155. 

 

 

2012

 

11. Biggar, K.K., Dawson, N. and Storey, K.B. (2012) Real-time protein unfolding: a method for determining the kinetics of native protein denaturation using a qRT thermocycler. Biotechniques. 53(4): 231-238. 

 

10. Biggar, K.K., Kornfeld, S.F. and Storey, K.B. (2012) Suppression of muscle disuse atrophy during mammalian hibernation: MicroRNA regulation in the skeletal muscle of Myotis lucifugus. Genom. Proteom. Bioinform. 10(5): 295-301. 

 

9. Biggar, K.K., Kornfeld, S.F., Maistrovski, Y. and Storey, K.B. (2012) Differential expression of miRNAs in the intertidal snail Littorina littorea during freezing and anoxia in foot muscle and hepatopancreas. Genom. Proteom. Bioinform. 10(5): 302-309. 

 

8. Maistrovski, Y., Biggar, K.K. and Storey, K.B. (2012) The conserved response of HIF-1α regulation in mammalian hibernators: examining the regulation of HIF-1α by long non-coding RNAs in the thirteen lined ground squirrel (Spermophilus tridecemlineatus) and little brown bat (Myotis lucifugus). J. Comp. Physiol. B, 182(6): 849-859. 

 

7. Biggar, K.K. and Storey, K.B. (2012) Evidence for cell cycle suppression and microRNA regulation of cyclin D1 during anoxia survival in turtles. Cell Cycle 11(9): 1705-1713. 

 

6. Biggar, K.K., Groom, A. and Storey, K.B. (2012) Hypometabolism in turtles: Physiological and molecular strategies of anoxia survival. In Hypometabolism: Strategies of survival in vertebrates and invertebrates (eds. Nowakowska, A. and Caputa, M.) Research Signpost. ISBN: 978-81-308-0471-2. 

 

 

2011

 

5. Biggar, K.K., Kornfeld, S.F. and Storey, K.B. (2011) Amplification and sequencing of mature microRNAs in uncharacterized animal models using stem-loop reverse transcription-polymerase chain reaction. Anal. Biochem. 416(2):231-233. 

 

4. Roufayel, R., Biggar, K.K. and Storey, K.B. (2011) Regulation of cell cycle components during exposure to anoxic and dehydration stress in the wood frog, Rana sylvatica. J. Exp. Zool. 315A(8):487-494. 

 

3. Biggar, K.K. and Storey, K.B. (2011) The emerging roles of microRNAs in the molecular responses of metabolic rate depression. J. Mol. Cell Biol. 3(3): 167-175. 

 

 

2009

 

2. Biggar, K.K. and Storey, K.B. (2009) Perspectives in cell cycle regulation: Lessons from an anoxic vertebrate. Current Genomics, 10(8): 573-584. 

 

1. Biggar, K.K., Dubuc, A., and Storey, K.B. (2009) MicroRNA regulation below zero: Differential expression of miRNA-21 and miRNA-16 during freezing in wood frogs. Cryobiology 59: 317–321. 

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